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Issue 17, 2019
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Ultrasensitive amplification-free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles

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Abstract

A single-particle enumeration method based on phosphorylation-directed in situ assembly of gold nanoparticles is developed for the ultrasensitive sensing of cellular protein kinase A activity. In comparison to existing strategies, the proposed new method demonstrates five orders of linear range and improves the detection limit up to 10-to-1000 fold without the involvement of target amplification.

Graphical abstract: Ultrasensitive amplification-free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles

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Publication details

The article was received on 06 Jan 2019, accepted on 30 Jan 2019 and first published on 30 Jan 2019


Article type: Communication
DOI: 10.1039/C9CC00131J
Citation: Chem. Commun., 2019,55, 2505-2508

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    Ultrasensitive amplification-free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles

    T. Tian, Y. Yao, B. Yang, K. Zhang and B. Liu, Chem. Commun., 2019, 55, 2505
    DOI: 10.1039/C9CC00131J

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