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Issue 15, 2019
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A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ

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Abstract

A semisynthetic fluorescent protein assembly-based FRET probe (sFPAP) was proposed for cell membrane protease function assay. Here, the probe was anchored on a living cell membrane through a membrane inserting peptide (MIP) and was successfully utilized for in situ imaging of furin activity on the living cell membrane in real time.

Graphical abstract: A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ

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Publication details

The article was received on 04 Dec 2018, accepted on 23 Jan 2019 and first published on 24 Jan 2019


Article type: Communication
DOI: 10.1039/C8CC09634A
Citation: Chem. Commun., 2019,55, 2218-2221

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    A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ

    S. Sun, Y. Liu, J. Xia, M. Wang, R. Tang, C. Lei, Y. Huang, Z. Nie and S. Yao, Chem. Commun., 2019, 55, 2218
    DOI: 10.1039/C8CC09634A

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