Issue 96, 2018

High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues

Abstract

We developed a fluorescent pH probe (1) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide–piperazine–rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues.

Graphical abstract: High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues

Supplementary files

Article information

Article type
Communication
Submitted
04 Oct 2018
Accepted
06 Nov 2018
First published
07 Nov 2018

Chem. Commun., 2018,54, 13531-13534

High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues

M. J. Chang, K. Kim, K. S. Park, J. S. Kang, C. S. Lim, H. M. Kim, C. Kang and M. H. Lee, Chem. Commun., 2018, 54, 13531 DOI: 10.1039/C8CC07934J

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