Noncovalent spin-labeling of RNA: the aptamer approach

Subham Saha; Thilo Hetzke; Thomas F. Prisner; Snorri Th. Sigurdsson

doi:10.1039/C8CC05597A

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Noncovalent spin-labeling of RNA: the aptamer approach†

Subham Saha,^a Thilo Hetzke,^b Thomas F. Prisner

^b and Snorri Th. Sigurdsson

*^a

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* Corresponding authors

^a Department of Chemistry, Science Institute, Dunhaga 3, 107 Reykjavik, Iceland
E-mail: snorrisi@hi.is

^b Institute of Physical and Theoretical Chemistry, J. W. Goethe University, Max-von-Laue-Str. 7, 60438 Frankfurt, Germany

Abstract

In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron–electron double resonance (PELDOR) and fluorescence spectroscopies.

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Article information

DOI: https://doi.org/10.1039/C8CC05597A
Article type: Communication
Submitted: 11 Jul 2018
Accepted: 24 Sep 2018
First published: 02 Oct 2018

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Chem. Commun., 2018,54, 11749-11752

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Noncovalent spin-labeling of RNA: the aptamer approach

S. Saha, T. Hetzke, T. F. Prisner and S. Th. Sigurdsson, Chem. Commun., 2018, 54, 11749 DOI: 10.1039/C8CC05597A

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Chemical Communications

Noncovalent spin-labeling of RNA: the aptamer approach†

Abstract

Supplementary files

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Noncovalent spin-labeling of RNA: the aptamer approach

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