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Issue 68, 2018
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Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

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Abstract

ortho-Phthalaldehyde (OPA) phosphoramidite with high reaction activity was designed and synthesized for labelling oligodeoxynucleotides (DNA). The DNA modified with OPA (OPA-DNA) can covalently couple with native proteins rapidly and efficiently via a condensation reaction with the formation of phthalimidines, which provides a highly efficient method for bioconjugation of DNA and native proteins under physiological conditions.

Graphical abstract: Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

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Publication details

The article was received on 26 Jun 2018, accepted on 30 Jul 2018 and first published on 30 Jul 2018


Article type: Communication
DOI: 10.1039/C8CC05037F
Citation: Chem. Commun., 2018,54, 9434-9437

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    Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

    Y. Ma, Z. Lv, T. Li, T. Tian, L. Lu, W. Liu, Z. Zhu and C. Yang, Chem. Commun., 2018, 54, 9434
    DOI: 10.1039/C8CC05037F

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