Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Fan Chen; Kecheng Li; Gene Hart-Smith; You Dan Xu; Yanyan Jiang; Hongxu Lu; Sandra Fok; Alexander Macmillian; Elvis Pandzic; Martina Stenzel

doi:10.1039/C8CC04986F

Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery†

Fan Chen,^a Kecheng Li,^a Gene Hart-Smith,^b You Dan Xu,^a Yanyan Jiang,^c Hongxu Lu,

^a Sandra Fok,^d Alexander Macmillian,^d Elvis Pandzic^d and Martina Stenzel

*^a

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* Corresponding authors

^a Centre for Advanced Macromolecular Design (CAMD), School of Chemistry, University of New South Wales, Sydney, New South Wales 2052, Australia
E-mail: M.Stenzel@unsw.edu.au

^b Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia

^c Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

^d Biomedical Imaging Facility, University of New South Wales, Sydney, New South Wales 2052, Australia

Abstract

Polyelectrolyte–protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

Chemical Communications

Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery†

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Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

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