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Issue 70, 2018
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ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions

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Abstract

We describe a new type of nucleotide-derived fluorescent probe designed for monitoring pyrophosphatase activity based on excimer-to-monomer transitions, called ExciTide. The nucleotides were designed with two self-interacting dye moieties and synthesised using copper-catalysed azide–alkyne cycloaddition click chemistry. We applied these probes for enzyme activity monitoring and inhibitor evaluation. Some of the probes permeated into living cells, yielding interesting prospects for future applications.

Graphical abstract: ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions

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Publication details

The article was received on 21 Jun 2018, accepted on 06 Aug 2018 and first published on 06 Aug 2018


Article type: Communication
DOI: 10.1039/C8CC04968H
Citation: Chem. Commun., 2018,54, 9773-9776

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    ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions

    P. Wanat, R. Kasprzyk, M. Kopcial, P. J. Sikorski, D. Strzelecka, J. Jemielity and J. Kowalska, Chem. Commun., 2018, 54, 9773
    DOI: 10.1039/C8CC04968H

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