Issue 75, 2018

A novel exonuclease-assisted isothermal nucleic acid amplification with ultrahigh specificity mediated by full-length Bst DNA polymerase

Abstract

Here, we introduced a novel exonuclease-assisted isothermal nucleic acid amplification (Exo-NAT) utilizing the full-length Bst DNA polymerase combined with a melting curve analysis. This method achieved an ultrahigh specificity with a good detection limit (102 copies) in both singleplex and multiplex detection, which was validated by detecting three diarrhea-inducing pathogens, rotavirus A, astrovirus, and adenovirus, in 42 clinical samples.

Graphical abstract: A novel exonuclease-assisted isothermal nucleic acid amplification with ultrahigh specificity mediated by full-length Bst DNA polymerase

Supplementary files

Article information

Article type
Communication
Submitted
08 Jun 2018
Accepted
19 Jul 2018
First published
19 Jul 2018

Chem. Commun., 2018,54, 10562-10565

A novel exonuclease-assisted isothermal nucleic acid amplification with ultrahigh specificity mediated by full-length Bst DNA polymerase

X. Ye, Y. Li, L. Wang, X. Fang and J. Kong, Chem. Commun., 2018, 54, 10562 DOI: 10.1039/C8CC04577A

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