Issue 65, 2018

Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin

Abstract

Substrate binding assays, in vitro proteolytic processing assays, and heterologous lasso peptide production were used to investigate the roles of conserved precursor peptide residues during paeninodin maturation. Specifically, we delineate which residues are important for substrate recognition, proteolysis, and lasso peptide macrocyclization.

Graphical abstract: Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin

Supplementary files

Article information

Article type
Communication
Submitted
01 Jun 2018
Accepted
20 Jul 2018
First published
20 Jul 2018

Chem. Commun., 2018,54, 9007-9010

Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin

J. D. Hegemann, C. J. Schwalen, D. A. Mitchell and W. A. van der Donk, Chem. Commun., 2018, 54, 9007 DOI: 10.1039/C8CC04411B

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