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Issue 57, 2018
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Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

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Abstract

Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

Graphical abstract: Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

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Article information


Submitted
06 Apr 2018
Accepted
15 May 2018
First published
22 May 2018

Chem. Commun., 2018,54, 7892-7895
Article type
Communication
Author version available

Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

K. Omura, Y. Aiba, H. Onoda, J. K. Stanfield, S. Ariyasu, H. Sugimoto, Y. Shiro, O. Shoji and Y. Watanabe, Chem. Commun., 2018, 54, 7892
DOI: 10.1039/C8CC02760A

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