Issue 84, 2017

Effect of buffer at nanoscale molecular recognition interfaces – electrostatic binding of biological polyanions

Abstract

We investigate the impact of an over-looked component on molecular recognition in water–buffer. The binding of a cationic dye to biological polyanion heparin is shown by isothermal calorimetry to depend on buffer (Tris–HCl > HEPES > PBS). The heparin binding of self-assembled multivalent (SAMul) cationic micelles is even more buffer dependent. Multivalent electrostatic molecular recognition is buffer dependent as a result of competitive interactions between the cationic binding interface and anions present in the buffer.

Graphical abstract: Effect of buffer at nanoscale molecular recognition interfaces – electrostatic binding of biological polyanions

Supplementary files

Article information

Article type
Communication
Submitted
22 Sep 2017
Accepted
05 Oct 2017
First published
09 Oct 2017

Chem. Commun., 2017,53, 11580-11583

Effect of buffer at nanoscale molecular recognition interfaces – electrostatic binding of biological polyanions

A. C. Rodrigo, E. Laurini, V. M. P. Vieira, S. Pricl and D. K. Smith, Chem. Commun., 2017, 53, 11580 DOI: 10.1039/C7CC07413A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements