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Issue 91, 2017
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Bypassing the proline/thiazoline requirement of the macrocyclase PatG

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Abstract

Biocatalysis is a fast developing field in which an enzyme's natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified two synthetic routes, triazole and a double cysteine, that circumvent this requirement. With the triazole, we show PatGmac can macrocyclise substrates that do not contain any amino acids in the final product.

Graphical abstract: Bypassing the proline/thiazoline requirement of the macrocyclase PatG

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Publication details

The article was received on 20 Aug 2017, accepted on 13 Sep 2017 and first published on 01 Nov 2017


Article type: Communication
DOI: 10.1039/C7CC06550G
Citation: Chem. Commun., 2017,53, 12274-12277
  • Open access: Creative Commons BY license
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    Bypassing the proline/thiazoline requirement of the macrocyclase PatG

    E. Oueis, H. Stevenson, M. Jaspars, N. J. Westwood and J. H. Naismith, Chem. Commun., 2017, 53, 12274
    DOI: 10.1039/C7CC06550G

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