Issue 81, 2017
From the journal:

Chemical Communications

Impact of cellular health conditions on the protein folding state in mammalian cells†

Kohsuke Inomata, ORCID logo *ab   Hajime Kamoshida,c   Masaomi Ikari,a   Yutaka Ito ORCID logo c  and  Takanori Kigawa ORCID logo *ad  

* Corresponding authors

a RIKEN Quantitative Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
E-mail: kinomata@riken.jp, kigawa@riken.jp

b PRESTO/Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

c Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan

d Department of Computer Science, School of Computing, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan

Abstract

By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.

Graphical abstract: Impact of cellular health conditions on the protein folding state in mammalian cells

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Article information

DOI
https://doi.org/10.1039/C7CC06004A
Article type
Communication
Submitted
01 Aug 2017
Accepted
22 Sep 2017
First published
22 Sep 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 11245-11248

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Impact of cellular health conditions on the protein folding state in mammalian cells

K. Inomata, H. Kamoshida, M. Ikari, Y. Ito and T. Kigawa, Chem. Commun., 2017, 53, 11245 DOI: 10.1039/C7CC06004A

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