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Issue 73, 2017
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A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases

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Abstract

K27-linkage poly-ubiquitination plays important roles in DNA damage repair and autoimmunity. Identification of K27-linkage targeting deubiquitinases (DUBs) is essential for understanding their regulatory mechanisms. Here we report an aryl-azide-based photoaffinity diubiquitin (diUb) probe for profiling K27-linkage targeting DUBs. This probe shows high selectivity and sensitivity towards K27-linkage DUBs in vitro. It can also be used in cell lysate for identifying and probing K27-linkage targeting DUBs in real proteomes. Our work suggests that the photoaffinity-based strategy may provide a new useful approach for the construction of other isopeptide linkage targeting DUB probes.

Graphical abstract: A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases

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Supplementary files

Article information


Submitted
16 Jul 2017
Accepted
22 Aug 2017
First published
22 Aug 2017

Chem. Commun., 2017,53, 10208-10211
Article type
Communication

A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases

X. Tan, M. Pan, S. Gao, Y. Zheng, J. Shi and Y. Li, Chem. Commun., 2017, 53, 10208
DOI: 10.1039/C7CC05504H

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