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Issue 63, 2017

Supercharging SpyCatcher toward an intrinsically disordered protein with stimuli-responsive chemical reactivity

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Abstract

We report a supercharged, intrinsically disordered protein, SpyCatcher(−), possessing stimuli-responsive reactivity toward SpyTag with tunable yields ranging from 4% to 98% depending on pH, temperature, ionic strength, etc. The CD and NMR studies reveal that the reaction occurs through a folded intermediate formed probably via a different mechanism from that of SpyCatcher.

Graphical abstract: Supercharging SpyCatcher toward an intrinsically disordered protein with stimuli-responsive chemical reactivity

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Article information


Submitted
10 Jun 2017
Accepted
28 Jun 2017
First published
28 Jun 2017

Chem. Commun., 2017,53, 8830-8833
Article type
Communication

Supercharging SpyCatcher toward an intrinsically disordered protein with stimuli-responsive chemical reactivity

Y. Cao, D. Liu and W. Zhang, Chem. Commun., 2017, 53, 8830 DOI: 10.1039/C7CC04507G

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