Issue 13, 2017

One-pot native chemical ligation by combination of two orthogonal thioester precursors

Abstract

We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. Combination of the two precursors facilitated the one-pot operation and yielded the entire polypeptide. The usefulness of this method was successfully demonstrated by the total synthesis of histone H4.

Graphical abstract: One-pot native chemical ligation by combination of two orthogonal thioester precursors

Supplementary files

Article information

Article type
Communication
Submitted
26 Dec 2016
Accepted
18 Jan 2017
First published
24 Jan 2017

Chem. Commun., 2017,53, 2114-2117

One-pot native chemical ligation by combination of two orthogonal thioester precursors

Y. Asahina, T. Kawakami and H. Hojo, Chem. Commun., 2017, 53, 2114 DOI: 10.1039/C6CC10243C

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