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Issue 6, 2017
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A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3′-terminal 2′-O-methylated plant microRNA

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Abstract

Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2′-O-methylated at the 3′ end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3′-terminal 2′-O-methylated miRNAs.

Graphical abstract: A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3′-terminal 2′-O-methylated plant microRNA

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Publication details

The article was received on 31 Oct 2016, accepted on 23 Dec 2016 and first published on 23 Dec 2016


Article type: Communication
DOI: 10.1039/C6CC08726D
Chem. Commun., 2017,53, 1124-1127

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    A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3′-terminal 2′-O-methylated plant microRNA

    X. Wang, H. Wang, C. Liu, H. Wang and Z. Li, Chem. Commun., 2017, 53, 1124
    DOI: 10.1039/C6CC08726D

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