Issue 84, 2016
From the journal:

Chemical Communications

Studies of G-quadruplexes formed within self-assembled DNA mini-circles†

Beata Klejevskaja,‡ab   Alice L. B. Pyne,‡c   Matthew Reynolds,‡a   Arun Shivalingam,a   Richard Thorogate,c   Bart W. Hoogenboom,cd   Liming Ying*be  and  Ramon Vilar ORCID logo *ab  

* Corresponding authors

a Department of Chemistry, Imperial College London, London, UK
E-mail: r.vilar@imperial.ac.uk

b Institute of Chemical Biology, Imperial College London, London, UK
E-mail: l.ying@imperial.ac.uk

c London Centre for Nanotechnology, University College London, London, UK

d Department of Physics and Astronomy, University College London, London, UK

e Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, UK

Abstract

We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.

Graphical abstract: Studies of G-quadruplexes formed within self-assembled DNA mini-circles

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Article information

DOI
https://doi.org/10.1039/C6CC07110D
Article type
Communication
Submitted
30 Aug 2016
Accepted
22 Sep 2016
First published
23 Sep 2016
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2016,52, 12454-12457

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Studies of G-quadruplexes formed within self-assembled DNA mini-circles

B. Klejevskaja, A. L. B. Pyne, M. Reynolds, A. Shivalingam, R. Thorogate, B. W. Hoogenboom, L. Ying and R. Vilar, Chem. Commun., 2016, 52, 12454 DOI: 10.1039/C6CC07110D

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