Issue 92, 2016
From the journal:

Chemical Communications

Hydrated ionic liquids as a liquid chaperon for refolding of aggregated recombinant protein expressed in Escherichia coli†

K. Fujita,‡*ab   M. Kajiyama,ab   Y. Liu,a   N. Nakamuraab  and  H. Ohno*ab  

* Corresponding authors

a Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan

b Functional Ionic Liquid Laboratories (FILL), Graduate School of Engneering, Tokyo University of Agriculture and Technology, Japan

Abstract

We have succeeded in refolding of aggregated recombinant protein from Escherichia coli in hydrated ionic liquids. In cholinium dihydrogen phosphate containing a limited amount of water molecules, aggregated solid cellulase was dissolved and refolding was successfully carried out without further processing.

Graphical abstract: Hydrated ionic liquids as a liquid chaperon for refolding of aggregated recombinant protein expressed in Escherichia coli

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Article information

DOI
https://doi.org/10.1039/C6CC06999A
Article type
Communication
Submitted
26 Aug 2016
Accepted
24 Oct 2016
First published
24 Oct 2016

Chem. Commun., 2016,52, 13491-13494

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Hydrated ionic liquids as a liquid chaperon for refolding of aggregated recombinant protein expressed in Escherichia coli

K. Fujita, M. Kajiyama, Y. Liu, N. Nakamura and H. Ohno, Chem. Commun., 2016, 52, 13491 DOI: 10.1039/C6CC06999A

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