Issue 99, 2016
From the journal:

Chemical Communications

Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein†

Shogo Tsuji,a   Shiroh Futakia  and  Miki Imanishi*a  

* Corresponding authors

a Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan
E-mail: imiki@scl.kyoto-u.ac.jp

Abstract

A 5mC-selective TALE-repeat was created by screening a TALE repeat library containing randomized amino acids at repeat variable diresidues and their neighboring residues. The new repeat showed high 5mC discrimination ability. An artificial TALE containing the new repeat activated an endogenous gene in a genomic methylation status-dependent manner.

Graphical abstract: Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein

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Article information

DOI
https://doi.org/10.1039/C6CC06824C
Article type
Communication
Submitted
19 Aug 2016
Accepted
16 Nov 2016
First published
16 Nov 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 14238-14241

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Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein

S. Tsuji, S. Futaki and M. Imanishi, Chem. Commun., 2016, 52, 14238 DOI: 10.1039/C6CC06824C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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