Issue 43, 2016
From the journal:

Chemical Communications

Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo†

Anyanee Kamkaew,‡a   Haiyan Sun,‡a   Christopher G. England,a   Liang Cheng,ab   Zhuang Liub  and  Weibo Cai*ac  

* Corresponding authors

a Departments of Radiology and Medical Physics, University of Wisconsin-Madison, WI 53705, USA
E-mail: wcai@uwhealth.org

b Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China

c University of Wisconsin Carbone Cancer Center, Madison, WI 53705, USA

Abstract

A small luciferase protein (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair. The conjugate showed 76% BRET efficiency and lymph node mapping was successfully performed. The cRGD peptide was conjugated to QD-Nluc for tumor targeting. The self-illuminating QD-Nluc showed excellent energy transfer in a living system and offered an optimal tumor-to-background ratio (>85).

Graphical abstract: Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C6CC02764D
Article type
Communication
Submitted
04 Apr 2016
Accepted
28 Apr 2016
First published
28 Apr 2016

Chem. Commun., 2016,52, 6997-7000

Permissions

Request permissions

Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo

A. Kamkaew, H. Sun, C. G. England, L. Cheng, Z. Liu and W. Cai, Chem. Commun., 2016, 52, 6997 DOI: 10.1039/C6CC02764D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements