Issue 40, 2016
From the journal:

Chemical Communications

Labeling of target mRNAs using a photo-reactive microRNA probe†

Kosuke Nakamoto,a   Koichiro Minami,b   Yukihiro Akaob  and  Yoshihito Ueno*ac  

* Corresponding authors

a United Graduate School of Agricultural Science, Gifu University, Japan
E-mail: uenoy@gifu-u.ac.jp

b United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan

c Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan

Abstract

To identify target mRNAs of an miRNA, we synthesized photo-reactive miRNA probes, which contained a photo-reactive nucleoside analog, 1-O-[4-(3-trifluoromethyl-3H-diazirine-3-yl)]benzyl-β-D-ribofuranose, in the middle of the strand. The photo-reactive miRNA-145 probe was found to specifically label the target mRNAs, FSCN1 and KLF4, by UV-A irradiation in human colon cancer DLD-1 cells.

Graphical abstract: Labeling of target mRNAs using a photo-reactive microRNA probe

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C6CC01360K
Article type
Communication
Submitted
16 Feb 2016
Accepted
19 Apr 2016
First published
19 Apr 2016

Chem. Commun., 2016,52, 6720-6722

Permissions

Request permissions

Labeling of target mRNAs using a photo-reactive microRNA probe

K. Nakamoto, K. Minami, Y. Akao and Y. Ueno, Chem. Commun., 2016, 52, 6720 DOI: 10.1039/C6CC01360K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements