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Issue 31, 2016
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Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

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Abstract

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

Graphical abstract: Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

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Supplementary files

Article information


Submitted
11 Jan 2016
Accepted
11 Mar 2016
First published
11 Mar 2016

Chem. Commun., 2016,52, 5467-5469
Article type
Communication

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

F. Caschera, J. W. Lee, K. K. Y. Ho, A. P. Liu and M. C. Jewett, Chem. Commun., 2016, 52, 5467
DOI: 10.1039/C6CC00223D

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