Issue 17, 2016
From the journal:

Chemical Communications

Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins†

Yun Zhang,*a   Jiani Yang,a   Jinfang Nie,*a   Juanhua Yang,a   Dong Gao,a   Lang Zhanga  and  Jianping Lia  

* Corresponding authors

a Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, P. R. China
E-mail: zy@glut.edu.cn, Niejinfang@glut.edu.cn

Abstract

This work describes a general methodology for enhanced enzyme-linked immunosorbent assay (ELISA) that integrates enzyme-coated microparticle probes for robust yet highly efficient signal amplification and a handheld pH meter for a simple, portable, quantitative readout. Its utility is well demonstrated with the detection of the target protein with a 14-fold enhancement of sensitivity in comparison with the conventional optical ELISA.

Graphical abstract: Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

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Article information

DOI
https://doi.org/10.1039/C5CC09852A
Article type
Communication
Submitted
29 Nov 2015
Accepted
09 Dec 2015
First published
09 Dec 2015

Chem. Commun., 2016,52, 3474-3477

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Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

Y. Zhang, J. Yang, J. Nie, J. Yang, D. Gao, L. Zhang and J. Li, Chem. Commun., 2016, 52, 3474 DOI: 10.1039/C5CC09852A

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