Jump to main content
Jump to site search

Issue 6, 2016
Previous Article Next Article

Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection

Author affiliations

Abstract

We develop a sensitive and selective method for the multiplex detection of histone-modifying enzymes (HMEs) through the integration of antibody-based fluorescence labeling with total internal reflection fluorescence (TIRF)-based single-molecule detection. This method exhibits excellent specificity and high sensitivity with a detection limit of 21 pM for histone acetyltransferase GcN5 and 12 pM for histone methyltransferase G9a, and it can be applied for the screening of HME inhibitors as well.

Graphical abstract: Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Oct 2015, accepted on 17 Nov 2015 and first published on 17 Nov 2015


Article type: Communication
DOI: 10.1039/C5CC08797J
Chem. Commun., 2016,52, 1218-1221

  •   Request permissions

    Multiplex detection of histone-modifying enzymes by total internal reflection fluorescence-based single-molecule detection

    F. Ma, M. Liu, Z. Wang and C. Zhang, Chem. Commun., 2016, 52, 1218
    DOI: 10.1039/C5CC08797J

Search articles by author

Spotlight

Advertisements