Issue 4, 2016

The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

Abstract

We developed a novel strategy by oxidation–derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.

Graphical abstract: The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

Supplementary files

Article information

Article type
Communication
Submitted
02 Sep 2015
Accepted
03 Nov 2015
First published
03 Nov 2015

Chem. Commun., 2016,52, 737-740

Author version available

The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

H. Zhang, J. Xiong, B. Qi, Y. Feng and B. Yuan, Chem. Commun., 2016, 52, 737 DOI: 10.1039/C5CC07354E

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