Photocatalytic oxidation of TMB with the double stranded DNA–SYBR Green I complex for label-free and universal colorimetric bioassay†
Abstract
We report here the newly discovered photocatalytic activity of the dsDNA–SYBR Green I (SG) complex, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) under light irradiation corresponding to the excitation of the dsDNA–SG complex. The most appealing feature of the photocatalytic system here is that it can be obtained using random DNA sequences that can form a duplex. Considering the universality of the photooxidase, a label-free and universal platform was proposed for highly sensitive visual bioassays.