A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds

Yu-Jing Lu; Qiang Deng; Dong-Ping Hu; Zheng-Ya Wang; Bao-Hua Huang; Zhi-Yun Du; Yan-Xiong Fang; Wing-Leung Wong; Kun Zhang; Cheuk-Fai Chow

doi:10.1039/C5CC05551B

A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds†

Yu-Jing Lu,*^a Qiang Deng,^a Dong-Ping Hu,^a Zheng-Ya Wang,^a Bao-Hua Huang,^a Zhi-Yun Du,^a Yan-Xiong Fang,^a Wing-Leung Wong,

*^b Kun Zhang*^ac and Cheuk-Fai Chow^b

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* Corresponding authors

^a Institute of Natural Medicine and Green Chemistry, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, P. R. China
E-mail: luyj@gdut.edu.cn, kzhang@gdut.edu.cn
Tel: +86-20-39322235

^b Department of Science and Environmental Studies, Centre for Education in Environmental Sustainability, The Hong Kong Institute of Education, 10 Lo Ping Road, Tai Po, Hong Kong SAR, P. R. China
E-mail: wingleung@ied.edu.hk
Tel: +852-2948-8401

^c School of Chemical and Environment Engineering, Wuyi University, Jiangmen 529020, P. R. China

Abstract

A new RNA-selective fluorescent dye integrated with a thiazole orange and a p-(methylthio)styryl moiety shows better nucleolus RNA staining and imaging performance in live cells than the commercial stains. It also exhibits excellent photostability, cell tolerance, and counterstain compatibility with 4′,6-diamidino-2-phenylindole for specific RNA–DNA colocalization in bioassays.

Chemical Communications

A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds†

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A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds

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