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Issue 69, 2015
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DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

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Abstract

The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated. Based on this unique quenching effect and the DNA-mediated ScGFP/GO interaction, a label-free fluorescence method has been established for homogeneously assaying the activity and inhibition of base excision repair enzyme.

Graphical abstract: DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

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Article information


Submitted
09 Jun 2015
Accepted
14 Jul 2015
First published
14 Jul 2015

Chem. Commun., 2015,51, 13373-13376
Article type
Communication
Author version available

DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

Z. Wang, Y. Li, L. Li, D. Li, Y. Huang, Z. Nie and S. Yao, Chem. Commun., 2015, 51, 13373
DOI: 10.1039/C5CC04759E

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