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Issue 61, 2015
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Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction

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Abstract

We develop a ligation-mediated polymerase chain reaction (PCR) for real-time detection of small biological molecules in a high-throughput format. This method is extremely sensitive with a detection limit of as low as 18.8 fM for ATP and 17.3 fM for NAD+, and it can discriminate target molecules from their analogues as well.

Graphical abstract: Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction

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Article information


Submitted
24 May 2015
Accepted
22 Jun 2015
First published
22 Jun 2015

Chem. Commun., 2015,51, 12270-12273
Article type
Communication

Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction

Y. Zhang, J. Liu and C. Zhang, Chem. Commun., 2015, 51, 12270
DOI: 10.1039/C5CC04269K

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