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Issue 72, 2015
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Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

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Abstract

Gd3+ is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd3+-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.

Graphical abstract: Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

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Article information


Submitted
22 May 2015
Accepted
22 Jul 2015
First published
22 Jul 2015

This article is Open Access

Chem. Commun., 2015,51, 13850-13853
Article type
Communication

Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

F. Wojciechowski, A. Groß, I. T. Holder, L. Knörr, M. Drescher and J. S. Hartig, Chem. Commun., 2015, 51, 13850
DOI: 10.1039/C5CC04234H

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