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Issue 54, 2015
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Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

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Abstract

Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

Graphical abstract: Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

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Supplementary files

Article information


Submitted
12 May 2015
Accepted
28 May 2015
First published
28 May 2015

Chem. Commun., 2015,51, 10953-10956
Article type
Communication
Author version available

Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Y. Wang, E. J. H. Wee and M. Trau, Chem. Commun., 2015, 51, 10953 DOI: 10.1039/C5CC03921E

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