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Issue 54, 2015
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An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2–ABD) as a recombinant target-specific signal amplifier

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Abstract

A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2–ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.

Graphical abstract: An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2–ABD) as a recombinant target-specific signal amplifier

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Supplementary files

Article information


Submitted
23 Mar 2015
Accepted
27 May 2015
First published
11 Jun 2015

Chem. Commun., 2015,51, 10945-10948
Article type
Communication
Author version available

An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2–ABD) as a recombinant target-specific signal amplifier

J. Lee, E. K. Song, Y. Bae, J. Min, H. Rhee, T. J. Park, M. Kim and S. Kang, Chem. Commun., 2015, 51, 10945
DOI: 10.1039/C5CC02409A

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