Issue 28, 2015
From the journal:

Chemical Communications

A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out†

Robert J. Meier,a   Johann M. B. Simbürger,a   Tero Soukkab  and  Michael Schäferling*c  

* Corresponding authors

a Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany

b Department of Biochemistry/Biotechnology, University of Turku, 20520 Turku, Finland

c BAM - Federal Institute for Materials Research and Testing, Division 1.10 Biophotonics, 12489 Berlin, Germany
E-mail: michael.schaeferling@bam.de

Abstract

A luminescent probe for determination of pH was designed based on a Förster resonance energy transfer (FRET) system, combining a europium chelate as the donor and carboxynaphtho-fluorescein as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.

Graphical abstract: A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out

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Article information

DOI
https://doi.org/10.1039/C5CC00144G
Article type
Communication
Submitted
07 Jan 2015
Accepted
26 Feb 2015
First published
26 Feb 2015

Chem. Commun., 2015,51, 6145-6148

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A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out

R. J. Meier, J. M. B. Simbürger, T. Soukka and M. Schäferling, Chem. Commun., 2015, 51, 6145 DOI: 10.1039/C5CC00144G

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