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We report the first synthesis of a methylene blue (MB) phosphoramidite derivative suitable for DNA solid-phase synthesis. The electrochemical and optical properties of the resulting MB modified oligonucleotides were confirmed. This new molecule is an important breakthrough in the design of new probes labelled with MB.
G. De Crozals, C. Farre, M. Sigaud, P. Fortgang, C. Sanglar and C. Chaix, Chem. Commun., 2015, 51, 4458 DOI: 10.1039/C4CC10164B
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