Issue 1, 2015

RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

Abstract

Cell-free transcription–translation systems offer an effective and versatile platform to explore the impact of genetic variations on protein function. We have developed a protocol for preparing linear, mutagenic DNA templates for direct use in the PURE system, enabling the fast and semi-quantitative evaluation of amino acid variations on catalytic activity and stereo-selectivity in native and engineered variants of Old Yellow Enzyme.

Graphical abstract: RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

Supplementary files

Article information

Article type
Communication
Submitted
17 Oct 2014
Accepted
04 Nov 2014
First published
04 Nov 2014

Chem. Commun., 2015,51, 122-124

RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

L. T. Quertinmont, R. Orru and S. Lutz, Chem. Commun., 2015, 51, 122 DOI: 10.1039/C4CC08240K

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