Issue 25, 2015
From the journal:

Chemical Communications

A simple bioluminescent method for measuring d-amino acid oxidase activity†

T. Spencer Bailey,‡a   Micah T. Donor,‡a   Sean P. Naughtona  and  Michael D. Pluth*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute, University of Oregon, Eugene, OR, USA
E-mail: pluth@uoregon.edu

Abstract

D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin.

Graphical abstract: A simple bioluminescent method for measuring d-amino acid oxidase activity

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Article information

DOI
https://doi.org/10.1039/C4CC08145E
Article type
Communication
Submitted
14 Oct 2014
Accepted
30 Oct 2014
First published
03 Nov 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 5425-5428

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A simple bioluminescent method for measuring D-amino acid oxidase activity

T. S. Bailey, M. T. Donor, S. P. Naughton and M. D. Pluth, Chem. Commun., 2015, 51, 5425 DOI: 10.1039/C4CC08145E

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