Issue 82, 2014
From the journal:

Chemical Communications

A click chemistry strategy for visualization of plant cell wall lignification†

Yuki Tobimatsu,‡§*a   Dorien Van de Wouwer,§bc   Eric Allen,a   Robert Kumpf,bc   Bartel Vanholme,bc   Wout Boerjanbc  and  John Ralph*a  

* Corresponding authors

a Department of Biochemistry and the US Department of Energy's Great Lakes Bioenergy Research Center (GLBRC), the Wisconsin Energy Institute, University of Wisconsin, Madison, WI, USA
E-mail: jralph@wisc.edu, yukitobi@kais.kyoto-u.ac.jp

b Department of Plant Systems Biology, VIB, Technologiepark 927, Gent, Belgium

c Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Gent, Belgium

Abstract

Bioorthogonal click chemistry was commissioned to visualize the plant cell wall lignification process in vivo. This approach uses chemical reporter-tagged monolignol mimics that can be metabolically incorporated into lignins and subsequently derivatized via copper-assisted or copper-free click reactions.

Graphical abstract: A click chemistry strategy for visualization of plant cell wall lignification

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Article information

DOI
https://doi.org/10.1039/C4CC04692G
Article type
Communication
Submitted
20 Jun 2014
Accepted
21 Aug 2014
First published
22 Aug 2014

Chem. Commun., 2014,50, 12262-12265

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A click chemistry strategy for visualization of plant cell wall lignification

Y. Tobimatsu, D. V. D. Wouwer, E. Allen, R. Kumpf, B. Vanholme, W. Boerjan and J. Ralph, Chem. Commun., 2014, 50, 12262 DOI: 10.1039/C4CC04692G

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