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Issue 69, 2014
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A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

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Abstract

A label-free double amplification system has been developed by using a ternary DNA probe containing the poly(adenine-thymine) sequence assisted by exonuclease III degradation. The method achieved more than 600-fold signal amplification and allowed sensitive detection of single-stranded DNA and thrombin at the pM level by using liquid chromatography/mass spectrometry.

Graphical abstract: A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

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The article was received on 12 May 2014, accepted on 09 Jul 2014 and first published on 09 Jul 2014


Article type: Communication
DOI: 10.1039/C4CC03559C
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Chem. Commun., 2014,50, 9846-9848

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    A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

    W. Zhao, Z. Qin, C. Zhang, M. Zhao and H. Luo, Chem. Commun., 2014, 50, 9846
    DOI: 10.1039/C4CC03559C

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