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Issue 66, 2014
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Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining β-glycosidases

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Abstract

Novel fluorogenic 2-deoxy-2-fluoroglycosyl acridinone active site titrating reagents were synthesised and kinetic parameters determined for their inactivation of two retaining β-glucosidases, a β-galactosidase, a β-xylosidase and several cellulases. Fluorescence-monitored active site titration using this class of reagents reliably measured active enzyme concentrations down to 3 nM.

Graphical abstract: Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining β-glycosidases

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Supplementary files

Article information


Submitted
03 May 2014
Accepted
04 Jun 2014
First published
30 Jun 2014

Chem. Commun., 2014,50, 9379-9382
Article type
Communication
Author version available

Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining β-glycosidases

T. Duo, E. D. Goddard-Borger and S. G. Withers, Chem. Commun., 2014, 50, 9379
DOI: 10.1039/C4CC03299C

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