Issue 94, 2014
From the journal:

Chemical Communications

Single-molecule dynamics of site-specific labeled transforming growth factor type II receptors on living cells†

Ming Cheng,a   Wei Zhang,a   Jinghe Yuan,a   Wangxi Luo,a   Nan Li,a   Shixian Lin,b   Yi Yang,b   Xiaohong Fang*a  and  Peng R. Chen*b  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P. R. China
E-mail: xfang@iccas.ac.cn

b Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, Synthetic and Functional Biomolecule Centre, Peking University, Beijing 100871, P. R. China
E-mail: pengchen@pku.edu.cn

Abstract

We achieved single-molecule imaging and tracking of the transforming growth factor type II receptor (TβRII) that was labeled by an organic dye via a genetically encoded unnatural amino acid (UAA) and the copper-free click chemistry. The stoichiometry, mobility and dimerization kinetics of individual TβRII molecules were determined.

Graphical abstract: Single-molecule dynamics of site-specific labeled transforming growth factor type II receptors on living cells

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Article information

DOI
https://doi.org/10.1039/C4CC02804J
Article type
Communication
Submitted
16 Apr 2014
Accepted
15 May 2014
First published
19 May 2014

Chem. Commun., 2014,50, 14724-14727

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Single-molecule dynamics of site-specific labeled transforming growth factor type II receptors on living cells

M. Cheng, W. Zhang, J. Yuan, W. Luo, N. Li, S. Lin, Y. Yang, X. Fang and P. R. Chen, Chem. Commun., 2014, 50, 14724 DOI: 10.1039/C4CC02804J

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