Issue 56, 2014
From the journal:

Chemical Communications

A genetically encoded aldehyde for rapid protein labelling†

Alfred Tuley,a   Yan-Jiun Lee,a   Bo Wu,a   Zhiyong U. Wangb  and  Wenshe R. Liu*a  

* Corresponding authors

a Department of Chemistry, Texas A&M University, College Station, TX, USA
E-mail: wliu@chem.tamu.edu

b Department of Chemistry and Physics, Troy University, Troy, Alabama 36081, USA

Abstract

Using a mutant pyrrolysyl-tRNA synthetase-tRNAPylCUA pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.

Graphical abstract: A genetically encoded aldehyde for rapid protein labelling

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4CC02000F
Article type
Communication
Submitted
17 Mar 2014
Accepted
04 Apr 2014
First published
09 Apr 2014

Chem. Commun., 2014,50, 7424-7426

Author version available

Download author version (PDF)

Permissions

Request permissions

A genetically encoded aldehyde for rapid protein labelling

A. Tuley, Y. Lee, B. Wu, Z. U. Wang and W. R. Liu, Chem. Commun., 2014, 50, 7424 DOI: 10.1039/C4CC02000F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements