Issue 25, 2014
From the journal:

Chemical Communications

Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification†

Cheng-Yi Hong,a   Xian Chen,a   Juan Li,a   Jing-Hua Chen,*b   Guonan Chena  and  Huang-Hao Yang*a  

* Corresponding authors

a The Key Lab of Analysis and Detection for Food Safety of MOE and Fujian Provincial, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China
E-mail: hhyang@fio.org.cn

b Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China
E-mail: cjh_huaxue@126.com

Abstract

We have developed a simple method for direct detection of circulating microRNAs in serum by using the p19 protein-functionalized magnetic beads (PFMBs) for specific enrichment and rolling circle amplification (RCA). The detection limit for microRNAs is 1 fM. Therefore, the proposed method has the potential of being used in the analysis of circulating microRNAs and clinical diagnosis.

Graphical abstract: Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification

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Article information

DOI
https://doi.org/10.1039/C3CC48996E
Article type
Communication
Submitted
25 Nov 2013
Accepted
03 Feb 2014
First published
03 Feb 2014

Chem. Commun., 2014,50, 3292-3295

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Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification

C. Hong, X. Chen, J. Li, J. Chen, G. Chen and H. Yang, Chem. Commun., 2014, 50, 3292 DOI: 10.1039/C3CC48996E

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