Functional isoDNA aptamers: modified thrombin binding aptamers with a 2′-5′-linked sugar-phosphate backbone (isoTBA)

Abstract

The regioisomeric 3′-deoxy-2′-5′-linked thrombin binding DNA aptamers (isoTBAs) were chemically synthesized and their ability to form unimolecular anti-parallel G-quadruplexes in the presence of K⁺ ions was evaluated. These modified sequences retain the function of the native thrombin binding aptamer (TBA), exhibit better stability against exonuclease and are capable of slowing down the process of blood clotting.