Issue 86, 2013
From the journal:

Chemical Communications

A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis†

Ching-Ching Yu,a   Li-De Huang,a   David H. Kwan,b   Warren W. Wakarchuk,c   Stephen G. Withers*b  and  Chun-Cheng Lin*a  

* Corresponding authors

a Department of Chemistry, National Tsing Hua University, 101, Sec. 2, Kuang Fu Rd., Hsinchu, Taiwan
E-mail: cclin66@mx.nthu.edu.tw
Fax: +886 3 5711082
Tel: +886 3 5753147

b Centre for High-throughput Biology (CHiBi) and Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada
E-mail: withers@chem.ubc.ca
Fax: +1 604 822 8869
Tel: +1 604 822 3402

c Department of Chemistry and Biology, Ryerson University, 350 Victoria St., Toronto, Ontario M5B 2K3, Canada

Abstract

We designed a novel strategy for sensitively detecting the activity of α-2,8-polysialyltransferase (PST) by a combination of ganglioside GD3 functionalized gold nanoparticles and inactive endosialidase. We anticipate that this new method will facilitate the search for PST inhibitors as well as for improved mutant forms of PST in directed evolution experiments.

Graphical abstract: A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

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Article information

DOI
https://doi.org/10.1039/C3CC45147J
Article type
Communication
Submitted
09 Jul 2013
Accepted
03 Sep 2013
First published
09 Sep 2013

Chem. Commun., 2013,49, 10166-10168

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A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

C. Yu, L. Huang, D. H. Kwan, W. W. Wakarchuk, S. G. Withers and C. Lin, Chem. Commun., 2013, 49, 10166 DOI: 10.1039/C3CC45147J

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