Issue 76, 2013
From the journal:

Chemical Communications

Real-time monitoring of DNA hybridization kinetics on living cell surfaces†

Ambadas B. Rode,a   Tamaki Endoh,a   Hisae Tateishi-Karimata,a   Shuntaro Takahashia  and  Naoki Sugimoto*ab  

* Corresponding authors

a Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047, Japan

b Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047, Japan
E-mail: sugimoto@konan-u.ac.jp
Fax: +81-78-303-1495
Tel: +81-78-303-1457

Abstract

We determined hybridization rates of DNA probes bound to the surfaces of a human cell, and compared the rates with those determined in solution. The rates were slower on the cell surface than in solution-phase. The position of the nucleation site but not the location of the formed duplex relative to the cell surface adversely affected the hybridization kinetics.

Graphical abstract: Real-time monitoring of DNA hybridization kinetics on living cell surfaces

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Article information

DOI
https://doi.org/10.1039/C3CC42990C
Article type
Communication
Submitted
23 Apr 2013
Accepted
30 May 2013
First published
30 May 2013

Chem. Commun., 2013,49, 8444-8446

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Real-time monitoring of DNA hybridization kinetics on living cell surfaces

A. B. Rode, T. Endoh, H. Tateishi-Karimata, S. Takahashi and N. Sugimoto, Chem. Commun., 2013, 49, 8444 DOI: 10.1039/C3CC42990C

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