Issue 15, 2013
From the journal:

Chemical Communications

Label-free electrochemical detection of an Entamoeba histolyticaantigen using cell-free yeast-scFv probes†

Yadveer S. Grewal,a   Muhammad J. A. Shiddiky,*a   Sean A. Gray,b   Kris M. Weigel,‡b   Gerard A. Cangelosi‡b  and  Matt Trau*a  

* Corresponding authors

a Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia
E-mail: m.shiddiky@uq.edu.au, m.trau@uq.edu.au
Fax: +61-7-33463973
Tel: +61-7-33464178

b Seattle Biomedical Research Institute, Seattle, WA 98117, USA

Abstract

Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.

Graphical abstract: Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

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Article information

DOI
https://doi.org/10.1039/C2CC38882K
Article type
Communication
Submitted
12 Dec 2012
Accepted
07 Jan 2013
First published
07 Jan 2013

Chem. Commun., 2013,49, 1551-1553

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Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

Y. S. Grewal, M. J. A. Shiddiky, S. A. Gray, K. M. Weigel, G. A. Cangelosi and M. Trau, Chem. Commun., 2013, 49, 1551 DOI: 10.1039/C2CC38882K

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