Issue 75, 2012
From the journal:

Chemical Communications

Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)†‡

Mhairi M. Harper,a   Barry Robertson,a   Alastair Rickettsb  and  Karen Faulds*a  

* Corresponding authors

a Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral St, Glasgow, UK
E-mail: Karen.faulds@strath.ac.uk
Tel: +44 (0)141 548 2507

b Renishaw Diagnostics Ltd, Nova Technology Park, Glasgow, UK

Abstract

We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.

Graphical abstract: Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2CC34859D
Article type
Communication
Submitted
07 Jul 2012
Accepted
01 Aug 2012
First published
14 Aug 2012

Chem. Commun., 2012,48, 9412-9414

Permissions

Request permissions

Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS)

M. M. Harper, B. Robertson, A. Ricketts and K. Faulds, Chem. Commun., 2012, 48, 9412 DOI: 10.1039/C2CC34859D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements