Issue 62, 2012
From the journal:

Chemical Communications

DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles†

Shiva K. Rastogi,*ab   CharLene M. Gibson,a   Josh R. Branen,a   D. Eric Aston,c   A. Larry Branena  and  Patrick J. Hrdlicka*b  

* Corresponding authors

a Biosensors and Nanotechnology Applications Laboratory, University of Idaho, Coeur d’Alene, USA
E-mail: srastogi@uidaho.edu

b Department of Chemistry, University of Idaho, Moscow, USA
E-mail: hrdlicka@uidaho.edu

c Department of Chemical and Materials Engineering, University of Idaho, Moscow, USA

Abstract

A lateral flow test strip assay, enabling sensitive detection of DNA specific to the foodborne pathogen E. coli O157:H7, is described. The use of LNA-conjugated gold nanoparticle probes, along with signal amplification protocols, results in minimum detectable concentrations of ∼0.4 nM.

Graphical abstract: DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles

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Article information

DOI
https://doi.org/10.1039/C2CC33430E
Article type
Communication
Submitted
11 May 2012
Accepted
11 Jun 2012
First published
13 Jun 2012

Chem. Commun., 2012,48, 7714-7716

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DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles

S. K. Rastogi, C. M. Gibson, J. R. Branen, D. Eric Aston, A. Larry Branen and P. J. Hrdlicka, Chem. Commun., 2012, 48, 7714 DOI: 10.1039/C2CC33430E

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