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Issue 50, 2012
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A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension

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Abstract

A novel electrochemical biosensor was developed for activity assay of DNA methyltransferase and its inhibitor based on methylation-sensitive cleavage, which activated a primer for terminal transferase-mediated extension of biotinylated dUTP followed by sensitive detection via enzymatic amplification.

Graphical abstract: A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension

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Publication details

The article was received on 03 Apr 2012, accepted on 01 May 2012 and first published on 01 May 2012


Article type: Communication
DOI: 10.1039/C2CC32397D
Chem. Commun., 2012,48, 6280-6282

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    A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension

    H. Wu, S. Liu, J. Jiang, G. Shen and R. Yu, Chem. Commun., 2012, 48, 6280
    DOI: 10.1039/C2CC32397D

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